Evaluation of the anti-oxidative and ROS scavenging properties of biomaterials coated with epigallocatechin gallate for tissue engineering

In tissue engineering, excessively generated reactive oxygen species (ROS) during biomaterial implantation or cell transplantation is a one of major causes diminishing therapeutic effects. this study, we prepared surfaces coated with antioxidant epigallocatechin gallate (EGCG) and metal ions, evaluated their anti-oxidative ROS scavenging properties. We revealed that EGCG-coating on polycaprolactone (PCL) film surface increased hydrophilicity properties as function total phenol content (TPC) potentially due to the increase in phenolic -OH ?-electrons from structural maintenance directly removed hydrogen peroxide (H2O2) by resonance-stabilization. Furthermore, EGCG-coated PCL attachment, spreading area, viability human adipose-derived stem cells (hADSCs) against H2O2 treatment while stimulated cellular signaling reduce apoptotic gene enhance enzyme expression. Further, applied EGCG coating poly-L-lactic acid (PLLA) fibers. Spheroids incorporating PLLA fibers were able maintain shape showed improved activities response H2O2-induced oxidative stress than control spheroids. Therefore, metal-phenolic network (MPN) suitable method impart biomaterials evaluating biological